● 產品概況/ product introduction
- 貨號EK0557
- 產品名稱Human ACE ELISA Kit
- 產品描述For quantitative detection of human ACE in cell culture supernates, serum, plasma(heparin) and saliva.
- 免疫原NSO, L30-L1261
- 交叉反應There is no detectable cross-reactivity with other relevant proteins.
- 敏感性＜5pg/ml
- 檢測范圍0.78ng/ml-50ng/ml
- 基因名稱ACE
- 基因全名angiotensin I converting enzyme
- Uniprot IDP12821
- 指標別名ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A
- 背景資料Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
- 顯色時間25-30分鐘
● 試劑盒內容/ product introduction
| SKU | 名稱 | 數量 |
| Catalog number | Description | Quantity |
| EK0557-CAP | 96-well plate precoated with anti- human ACE antibody | 1 |
| EK0557-ST | Lyophilized recombinant human ACE standard | 50ng/tubex2 |
| EK0557-DA | Biotinylated anti- human ACE antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| *小貼士：額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。 | ||
● 需要用戶自己提供的器材和試劑/ others
- ?96孔讀板儀器。
- ?自動洗板機。
- ?移液器和槍頭。建議使用多通道移液器/排槍。
- ?Eppendorf 管。
- ?額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
● 批內差/批間差/ others
| | Intra-Assay Precision | Inter-Assay Precision | ||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1278 | 6839 | 24157 | 1358 | 6350 | 25528 |
| Standard deviation | 62.62 | 465.05 | 1087.06 | 88.27 | 482.6 | 1225.34 |
| CV(%) | 4.90% | 6.80% | 4.50% | 6.50% | 7.60% | 4.80% |
